Composite

Part:BBa_K2921550:Design

Designed by: Allison Kuo   Group: iGEM19_TAS_Taipei   (2019-10-14)


Promoter + RBS + 6xHIS + Met + GS-linker + mRFP + Double Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 824
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1649
    Illegal AgeI site found at 1761
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 236


Design Notes

A GS-linker was added between the metallothionein and mRFP to fuse the two proteins. A colored protein was added to allow for visualization of the metal-binding protein.


Source

This part is made of the promoter BBa_K880005 with the part BBa_K1460002.

References